MSC Serum-free Medium (GMP grade)
Product name | Item No. | Packing specifications | Storage condition | Shelf life |
Serum-free minimal medium for mesenchymal stem cells (GMP grade) | NC0106 | 500 mL/bottle | 2-8°C, protected from light | 12 months |
MSC serum free medium additive (GMP grade) | NC0106.S | 5 mL/bottle | -20°C, protected from light | 12 months |
The inoculum size is provided below if petri dish of different surface area is used:
Culture flask/dish | Approximate bottom area (cm2) | Day 0 medium volume (mL) | Day 2 medium volume (mL) | Tissue segment added (number) |
60 mm dish | 21 | 2 | 3 | 5 |
100mm dish | 55 | 5 | 7 | 12 |
T-25 flask | 25 | 2 | 3 | 6 |
T-75 flask | 75 | 7 | 8 | 15 |
T-175 flask | 175 | 15 | 20 | 40 |
T-225 flask | 225 | 20 | 25 | 50 |
Cell Karyotype Detection
Chromosome coloring method: G banding | Banding standard:400-550 | Analyzing tool: CytoVision,Leica |
The number of detected cells:100 | Count the number of cells:100 | Karyotyping Cell Number:50 |
Test Result | ||
Karyotype analysis results of 100 dividing cells | ||
Chromosomal Analysis Project | Abnormal cell count | Reference |
Chromatids and chromosome breaks | 3 | ≤ 8 |
Abnormal structure | 1 | ≤ 2 |
Hyperdiploid | 0 | ≤ 2 |
Hypodiploid | 5 | ≤ 18 |
Polyploid | 0 | ≤ 4 |
